My colleague was doing the 1st part of the PCR last week. So this morning all i had to do was just the ethanol washing step. Plain simple. But when i look at the tube i think she did it wrongly because the supernantant was suprisingly alot. I wanted to ask her abt it, but i thought she has done it so many times which means it should be ok. So after doing my washing part, i incubate it. Later she saw the tube then ask me what i had added. I told her it was 60uL of buffer. Then she say cannot be because the amt in the tube is alot. I then show her the micro-pipette, it is 60uL what.. But i dont think she bought it. Pls lah...why would i want to lie to you. Its only the washing step, what else could i have added..My snort??? But anyway, she say she will take it from there. Better still, let her do it so she cant put the blame on others.
